Description
Intended Uses: The CA19-9 ELISA Kit is intended for the quantitative determination of the Cancer Antigen CA19-9 concentration in human serum or plasma. For research use only.
Principle of the Assay::The CA19-9 ELISA test is an adapted solid phase sequential sandwich ELISA. Samples and biotinylated monoclonal antibody are added to wells coated with streptavidin. CA19-9 in the patient sample binds to biotinylated capture antibody. The biotinylated antibody simultaneously binds to the streptavidin coated plate. After a wash step, anti-CA19-9-HRP enzyme conjugate is added and forms a sandwich around captured CA19-9. Unbound antibodies are washed off. TMB substrate is added resulting in the development of a blue color. The concentration of CA19-9 is directly proportional to the color intensity developed. A standard curve is generated relating color intensity to CA19-9 concentration